Diverse Cellular Functions of the Hsp90 Molecular Chaperone Uncovered Using Systems Approaches
Date
2007-10
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
A comprehensive understanding of the cellular
functions of the Hsp90 molecular chaperone
has remained elusive. Although Hsp90 is essential,
highly abundant under normal conditions,
and further induced by environmental stress,
only a limited number of Hsp90 ‘‘clients’’ have
been identified. To define Hsp90 function, a
panelofgenome-wide chemical-genetic screens
in Saccharomyces cerevisiae were combined
with bioinformatic analyses. This approach
identified several unanticipated functions of
Hsp90 under normal conditions and in response
to stress. Under normal growth conditions,
Hsp90 plays a major role in various aspects of
the secretory pathway and cellular transport;
during environmental stress, Hsp90 is required
for the cell cycle, meiosis, and cytokinesis. Importantly,
biochemical and cell biological analyses
validated several of these Hsp90-dependent
functions, highlighting the potential of our
integrated global approach to uncover chaperone
functions in the cell.
Description
item.page.type
Article
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Keywords
Heat shock proteins -- Analysis
Citation
Cell 131, 121–135, October 5, 2007 DOI 10.1016/j.cell.2007.07.036