Heterozygous Yeast Deletion Collection Screens Reveal Essential Targets of Hsp90

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dc.contributor.authorFranzosa, Eric A.
dc.contributor.authorAlbanèse, Véronique
dc.contributor.authorFrydman, Judith
dc.contributor.authorXia, Yu
dc.contributor.authorMcClellan, Amie J.
dc.date.accessioned2016-10-28T19:04:08Z
dc.date.available2016-10-28T19:04:08Z
dc.date.issued2011-11
dc.description.abstractHsp90 is an essential eukaryotic chaperone with a role in folding specific ‘‘client’’ proteins such as kinases and hormone receptors. Previously performed homozygous diploid yeast deletion collection screens uncovered broad requirements for Hsp90 in cellular transport and cell cycle progression. These screens also revealed that the requisite cellular functions of Hsp90 change with growth temperature. We present here for the first time the results of heterozygous deletion collection screens conducted at the hypothermic stress temperature of 15°C. Extensive bioinformatic analyses were performed on the resulting data in combination with data from homozygous and heterozygous screens previously conducted at normal (30°C) and hyperthermic stress (37°C) growth temperatures. Our resulting meta-analysis uncovered extensive connections between Hsp90 and (1) general transcription, (2) ribosome biogenesis and (3) GTP binding proteins. Predictions from bioinformatic analyses were tested experimentally, supporting a role for Hsp90 in ribosome stability. Importantly, the integrated analysis of the 15°C heterozygous deletion pool screen with previously conducted 30°C and 37°C screens allows for essential genetic targets of Hsp90 to emerge. Altogether, these novel contributions enable a more complete picture of essential Hsp90 functions.en_US
dc.description.sponsorshipThis work was supported by National Institutes of Health (NIH; www.nih.gov) grant GM56433 to JF, NIH R15 AREA grant GM087654-01 to AJM, National Science Foundation IGERT (http://www.igert.org/) grant DGE-0654108 to EAF, and Pharmaceutical Research and Manufacturers of America (PhRMA; http://www. phrmafoundation.org/) Foundation Research Starter Grant in Informatics to YX. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.identifier.citationFranzosa EA, Albane`se V, Frydman J, Xia Y, McClellan AJ (2011) Heterozygous Yeast Deletion Collection Screens Reveal Essential Targets of Hsp90. PLoS ONE 6(11): e28211. doi:10.1371/journal.pone.0028211en_US
dc.identifier.urihttp://hdl.handle.net/11209/10519
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.subjectYeast
dc.subjectEukaryotic cells
dc.subjectMolecular chaperones
dc.titleHeterozygous Yeast Deletion Collection Screens Reveal Essential Targets of Hsp90en_US
dc.typeArticleen_US

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